> At 01:26 PM 4/8/99 -0700, Michael Moore wrote:
> >Does anyone have a suggestion for measuring the amount of agragated vs.
> >non-agragated monoclonal antibody. The MW is approximately 150kD, and=
the
> >agragates are most likely greater than dimers. Thanks in advance
> >Michael
Something more exotic (I think you would have to build it yourself,=20
but I remember being impressed by the speed and at how effective the=20
technique was for _large_ aggregates) :
Litz=E9n A., Walter J.K., Krischollek H., Wahlund K.-G.
Separation and Quantitation of Monoclonal Antibody Aggregates by
Asymmetrical Flow Field-Flow Fractionation and Comparison to Gel
Permeation Chromatography 1993 Analyt.Biochem. 212 469-480
Patrick Corran