We have had both the LightCycler in the lab (about 2 years ago) and, more
recently, a couple of ABI 7700's ("TaqMan's"). The lightcycler has not been
used since we got the ABI machines in. I personally have not used the
Lightcycler to comment, but I have used the ABI machines, and have been very
impressed with the results. Its main advantage is definitely its
high-throughput aspect (2-3 hour run, 96 samples at a time, with the option
of doing two reactions/tube - see
http://www2.perkin-elmer.com/ab/about/pcr/sds/7700_SDS/7700.html).
Also, ABI are working on upgrades for the ABI7700 to produce, eventually,
chip-format loads for the machine.
So I suppose it depends on your needs as to which you decide to get.
Kind Regards,
Andrew Walding
_______________________________________________________________
ASTRA CHARNWOOD
Molecular Biology, Bakewell Road, Loughborough, Leics, ENGLAND LE11 5RH
Tel: +44 (0)1509 644213 Mobile: +44 (0)778 8595040 Fax: +44 (0)1509
645557
andrew.walding@charnwood.gb.astra.com
> ----------
> From: Susan San Francisco[SMTP:pxida@TTACS.TTU.EDU]
> Sent: 09 April 1999 17:11
> To: Recipients of ABRF List
> Subject: quantitative PCR
>
> Hi!
>
> A colleague has asked me about the Roche light cycler system for
> quantitative PCR. They are considering obtaining this instrument and
> wanted
> some opinions about it. I know nothing about it and have no direct
> experience with quantitative PCR, but I hoped someone in this group could
> give some advice/opinions.
> Thanks in advance for any information!
>
> Susan San Francisco
> pxida@ttacs.ttu.edu
> Texas Tech University
> Biotechnology Core Facility
>
>