> We've been monitoring aggregation of peptides (10-100kDa) by size exclusion
> chrom, but the process is proving to be too slow. I was encouraged by the
> positive responses about SE-HPLC. What columns are being used successfully?
>
> Thanks for your help!
>
> Jeni
>
> Janelle Lauer-Fields
> Department of Chemistry and Biochemistry
> Florida Atlantic University
> 777 Glades Road
> Boca Raton, FL 33431
> Phone: 561-297-2094
> FAX: 561-297-2759
Dear Jeni,
We have successfully used a FPLC fitted with a Superose column for
analysis of oligomer formation of enterolobin - a 53 kDa pore-forming protein.
Analysis time was as low as in any HPLC run. I hope this information is of any
help to you.
Good luck.
Marcelo.
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Prof. Marcelo Valle de Sousa
Brazilian Center for Protein Research and Services - CBSP
Laboratory of Protein Chemistry and Biochemistry
Departament of Cell Biology
University of Brasilia
Brasilia - DF Brasil
CEP 70910-900
Tel: (061)307-2142
Fax: (061)272-4548
e-mail: mvsousa@unb.br
http://www.unb.br/cbsp
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