RE: HPLC systems

Singleton, David H (david_h_singleton@groton.pfizer.com)
Thu, 15 Apr 1999 06:20:46 -0400

Something which isn't obvious, but is important: When choosing an HPLC for
purification, consider how it deals with fraction collection. Many systems
don't do well with chart marks; some have a slow A/D conversion rate making
real-time peak collection difficult necessitating a chart-recorder. Others,
especially PDA's, don't display meaningful data "on-the-fly" but write great
report methods.

Personally, I like my Beckman system from most standpoints. Chart marks
from a fraction collector required a separate A/D board, but it works.

It seems HPLC's are designed from a QC or stability application and
preparative techniques are an afterthought.

David H. Singleton
Scientist
Pfizer Central Research
PO Box 8003
Eastern Point Road
Groton, CT 06340-8003

-----Original Message-----
From: imbalzan@SAS.UPENN.edu [mailto:imbalzan@SAS.UPENN.edu]
Sent: Wednesday, April 14, 1999 3:24 PM
To: Recipients of ABRF List
Subject: HPLC systems

I plan to buy a new HPLC system to be used primarily for
synthetic oligo purification. I've been looking at systems from
Beckman, Waters, and HP. I was wondering if anyone had any comments
(good or bad) about the equipment they are currently using.

I am particularly interested in feedback on the HP1100.
I was recently told that they are not reliable.

Thanks so much,

Mike