PNG'ase F substrates

Higgins, Liz (lhiggins@Genzyme.com)
Thu, 15 Apr 1999 08:51:41 -0400

We were using RNase B as a substrate for measuring PNG'ase F activity but
Sigma is no longer selling it and I can't find an alternate source with a
preparation that isn't heavily contaminated with RNase A.

I'm looking for a glycoprotein with a single glycosylation site that is
commercially available (a tall order!). The only possibility I've found so
far is ovalbumin.

Does anybody have a candidate glycoprotein, or does anyone know of a good
source of RNase B?

Any help would be greatly appreciated!

Liz Higgins
-------------------------------------------------------------
Elizabeth Higgins, Ph.D.
Carbohydrate Chemistry
Genzyme Corporation
One Mountain Rd., Framingham, MA 01701-9322
508-270-2301
lhiggins@genzyme.com