Re: HPLC systems

Richard T. Pon (rtpon@ucalgary.ca)
Thu, 15 Apr 1999 09:16:14 -0600

Hi
I agree with the following message concerning fraction collection. For
prep work it is important to have an accurate and easy method to know
what each collection tube holds. We bought a Waters system about ten
years ago, which has been reliable, but the interface with our fraction
collector is quite "clunky". If I had to purchase a new system, I would
try to find a vendor who makes both parts of the system, i.e. the HPLC
and the fraction collector. To my knowledge, only Pharmacia's Akta line
combine both. It might be worth looking into, but I have had no direct
experience with them.

Singleton, David H wrote:
>
> Something which isn't obvious, but is important: When choosing an HPLC for
> purification, consider how it deals with fraction collection. Many systems
> don't do well with chart marks; some have a slow A/D conversion rate making
> real-time peak collection difficult necessitating a chart-recorder. Others,
> especially PDA's, don't display meaningful data "on-the-fly" but write great
> report methods.
>
> Personally, I like my Beckman system from most standpoints. Chart marks
> from a fraction collector required a separate A/D board, but it works.
>
> It seems HPLC's are designed from a QC or stability application and
> preparative techniques are an afterthought.
>
> David H. Singleton
> Scientist
> Pfizer Central Research
> PO Box 8003
> Eastern Point Road
> Groton, CT 06340-8003
>
> -----Original Message-----
> From: imbalzan@SAS.UPENN.edu [mailto:imbalzan@SAS.UPENN.edu]
> Sent: Wednesday, April 14, 1999 3:24 PM
> To: Recipients of ABRF List
> Subject: HPLC systems
>
> I plan to buy a new HPLC system to be used primarily for
> synthetic oligo purification. I've been looking at systems from
> Beckman, Waters, and HP. I was wondering if anyone had any comments
> (good or bad) about the equipment they are currently using.
>
> I am particularly interested in feedback on the HP1100.
> I was recently told that they are not reliable.
>
> Thanks so much,
>
> Mike

-- 
Richard T. Pon, University of Calgary
Professor, Adjunct, Dept. of Biochemistry & Molecular Biology and
Director, University Core DNA Services
3350 Hospital Drive N.W., Calgary, AB, Canada T2N 4N1
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283-4907


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