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Subject: PNG'ase F substrates
Author: ,"Higgins, Liz" <lhiggins@Genzyme.com> at INTERNET
Date: 4/15/99 12:10 PM
We were using RNase B as a substrate for measuring PNG'ase F activity but
Sigma is no longer selling it and I can't find an alternate source with a
preparation that isn't heavily contaminated with RNase A.
I'm looking for a glycoprotein with a single glycosylation site that is
commercially available (a tall order!). The only possibility I've found so
far is ovalbumin.
Does anybody have a candidate glycoprotein, or does anyone know of a good
source of RNase B?
Any help would be greatly appreciated!
Liz Higgins
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Elizabeth Higgins, Ph.D.
Carbohydrate Chemistry
Genzyme Corporation
One Mountain Rd., Framingham, MA 01701-9322
508-270-2301
lhiggins@genzyme.com