We recently had a complaint about HPLC purified oligonucleotides used for
sequencing. The hybridization versus the target seems OK but there is a
strong noise background on the electrophoregram making the reading
difficult. Do you think that triethylammonium or ammonium acetate salts
used for HPLC purification
could be responsible for this effect ? I don't know if this can be a
plausible explanation for unsuccessful sequencing ?
By the way do you know the main causes for bad results in sequencing ?
Thank You
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Alain LAURENT Ph. D
Oligonucleotide Chemistry
ESGS Groupe CYBERGENE
11 rue Claude Bernard
35400 SAINT MALO
FRANCE
Tel: +33 (0)2 99 21 90 40
Fax: +33 (0)2 99 21 90 41
e-mail: a.laurent@eurosequence.com
http://www.eurosequence.com
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