Just some quick thoughts slightly off-topic. For our in-house work I have found
Mallingkrodt HPLC grade acetonitrile (HPs L1) and methanol (HPs L2) work fine in the
G1005As, no problems. At around AUS $60 for 4 litres it's a huge saving. Also for our
in-house work I have found that used PVDF sample columns can be re-used with no
problems. You simply re-prep them using the PVDF 3.1 prep program and all pre-
existing protein is removed. I'm yet to attempt re-using the 3.1 protein columns but I
expect this can also be done. I'll be interested to hear how you go with your specific
problem. It may be the R2.
As I have said before I have a tremendous amount of respect for the HP sequencers.
My analogy is the HPs are like Harley Davidson's whereas the ABIs are like Hondas.
The 494s are more sensitive (they have an excellent initial yield) and are quicker, but if
you want a simple machine to mosy on down the highway in comfort take the Harley.
Jim Mackintosh
Australian Proteome Analysis Facility, Level 4, Building F7B, Macquarie University, Sydney, NSW, 2109, Australia
Tel: + 612 9850 6364 Fax: + 612 9850 6200 E-mail: jmackint@proteome.org.au http://www.proteome.org.au/