Regards
Andrew
_______________________________________________________________
ASTRA CHARNWOOD
Molecular Biology, Bakewell Road, Loughborough, Leics, ENGLAND LE11 5RH
Tel: +44 (0)1509 644213 Mobile: +44 (0)778 8595040 Fax: +44 (0)1509
645557
andrew.walding@charnwood.gb.astra.com
> ----------
> From: Njau, Reuben Kirobi[SMTP:rnjau@iupui.edu]
> Sent: 28 April 1999 14:14
> To: Recipients of ABRF List
> Subject: RE: Sequencing plates and plate cleaner
>
> I think 3 years is pushing those glass plates too far especially if they
> are
> used every day,it might be time to buy new ones. I have used the sigma
> plates, they are cheap and very good. One note though, most often the
> plates
> are back orderd and it might take some time before you get them. I have
> used
> both ABI's and Sigma and to me it does not make any difference as far as
> the
> data is concerned. You might also try liqui-Nox, to clean the plates this
> is
> what I use and it works very well.
>
> Good luck
>
> Reuben Njau
> Biochemistry Biotechnology Facility
> Indiana University School of Medicine
> Indianapolis, IN
> -----Original Message-----
> From: Skip Vaught, Brian Coullahan, Mark Schwartz, and Felisa Blackmer
> [mailto:jev@u.arizona.edu]
> Sent: Tuesday, April 27, 1999 4:34 PM
> To: Recipients of ABRF List
> Subject: Sequencing plates and plate cleaner
>
>
> Dear fellow sequencers,
>
> We're wondering if it's time replace our glass plates (377 standard,
> 36cm.). They've been used constantly for over 3 years. We are plagued
> occasionally by mobility problems in some lanes and the "disappearing -
> reappearing" bands. We have temporarily solved the problem by "acid/base"
> washing the plates. We have used Multiterge, Citrinox, Alconox, and
> Seque-soap to wash the plates, but the problem inevitably returns. (We
> understand a very hot DI water rinse works well, but we can't afford a lab
> dishwasher).
>
> My question is, does age "predispose" plates to this problem or do proper
> cleaning methods allow plates to be used indefinately?
>
> We are considering buying plates from Sigma. They're half the price of
> ABI's and their ad claims use by genome centers and core facilities
> worldwide! Are they comparable to ABI's?
>
> The latest issue of American Biotechnology Laboratory has an ad for a
> product called GELPLATE-clean. It claims that this product removes the
> contaminanting molecules on the glass surface without the need for
> soaking.
> Does anyone have experience with this product? Does it leave any
> fluorescent residue?
>
> Any advice is welcomed.
>
> Best regards,
>
> Skip Vaught
> DNA Sequencing Service
> University of Arizona
>
>
>
>
>
>
>
>
>
>
> THE DNA SEQUENCING SERVICE
> UNIVERSITY OF ARIZONA
>
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> Skip Vaught- jev@u.arizona.edu
> Brian Coullahan- coullaha@u.arizona.edu
> Mark Schwartz- schwartm@u.arizona.edu
> Amy Raymond- raymonda@u.arizona.edu
>