RE: Plate cleaning

Deb Grove (dsg4@psu.edu)
Fri, 30 Apr 1999 07:50:47 -0400

We have been using the same plates for a long time and do the
alconox/hotwater/deionized wash as well.
We do a light wipe with methanol using Kimpwipes EX-L before pouring.
No problems!

We ALWAYS use the same side of the notched plate because of the "memory" of
the rubber gasket on the plate from the upper buffer chamber.

Deb G.

>I have been using 373/377 for nearly 5 years, I always hand wash (no
>gloves) with alconox/Hot Water/De-ionised. I rarely spend more than 1-2
>minutes per plate and have never ever had any problems, even with plates
>that are 3 years old (both 36cm and 48cm plates). I do a acid/base wash of
>plates maybe once or twice a year .
>
>The only other thing we do is to get a double kim wipe lightly dipped in
>isopropanol and give a hard scrub of the inner plate surface just before
>pouring .This is something I was shown to do during our first 377 install
>after we had continual gel pouring problems due to uneven gel front while
>pouring , I do not know if this is a common practice or not but it works
>for me.
>
>I have to agree that there is a "mystery" factor to this problem that has
>not been properly accounted for.
>
>Kawasaki ZZR 600 |1992 Nissan Silvia | SR20DET | Bilstein shocks
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Deborah S. Grove, Ph.D.
Manager of the Nucleic Acid Facility
Biotechnology Institute
The Pennsylvania State University
210 Wartik Lab
University Park PA 16802

http://www.biotec.psu.edu/lsc/stf/naf/naf.html