PCR: quantitative
Mark Lively (mlively@wfubmc.edu)
Sat, 01 May 1999 11:54:54 -0400
ABRF discussion group:
A colleague at my institution has asked me for information regarding
quantitative PCR methods. He wishes to use quantitative PCR with tumor
specific primers to quantify the presence of human leukemia cells in
mouse blood in an animal model. I would appreciate receiving any input
on the methods and/or instrumentation used for this purpose. This
investigator asked specifically about the "5700 and 7700" machines which
I think must refer to instruments made by PE/ABI. He asks specifically
if they are "worth the cost."
Thanks in advance for your input.
-ml
> dear mark, i wish to follow the presence of human nucleated cells
> (leukemia cells) in mouse (scid mouse) blood. one approach would be
> to extract DNA and run a quantitative pcr using human specific-primers
> and probe with quencher and fluorochrome. does anyone have experience
> with the 5700 or 7700 machines for such applications and is it worth
> the cost/training? thanks,art
--
Mark O. Lively, Ph.D.
Professor of Biochemistry
Wake Forest University School of Medicine
Medical Center Blvd.
Winston-Salem, North Carolina 27157
Voice: 336-716-2969
Fax: 336-716-7200
email: mlively@wfubmc.edu