Re: Detection of Phosphorylated Proteins on Blots
Ken Mitchelhill (k.mitchelhill@medicine.unimelb.edu.au)
Mon, 3 May 1999 10:01:31 +1000
>Date: Mon, 3 May 1999 10:00:58 +1000
>To: grant.ra@pg.com
>From: Ken Mitchelhill <k.mitchelhill@medicine.unimelb.edu.au>
>Subject: Re: Detection of Phosphorylated Proteins on Blots
>Cc:
>Bcc:
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>Ray,
>
>this subject has been frequently discussed by the kinase email discussion
>group and I think the consensus is that, whilst there are non-specific
>PSer and PThr antibodies on the market, they are of little utility
>compared to their PTyr counterparts.
>
>I would however be delighted to hear from a laboratory that has had any of
>them work in their hands.
>
>Ken
>
>>I would like to detect phosphorylated proteins that have been separated by
>>2D gel electrophoresis and then blotted onto PVDF membranes. (These
>>proteins cannot be conveniently labelled with 32P in vivo.)
>>
>>What manufacturers' antibodies work best for detecting phosphotyrosine?
>>Are there any good ones for detecting phosphoserine/threonine??
>>
>>Thanks for all of your suggestions!
>>
>>Ray Grant
>>The Procter & Gamble Co.
>>Cincinnati, OH
>>
>>513-627-2179
>>grant.ra@pg.com
>
>
>
********************************
Ken I. Mitchelhill
The John Holt Protein Structure Laboratory
St. Vincent's Institute of Medical Research
41 Victoria Parade
Fitzroy 3065 Victoria
AUSTRALIA
Telephone: 61-3-9288 2480
Facsimile: 61-3-9416 2676
Email: k.mitchelhill@medicine.unimelb.edu.au
Laboratory: http://www.medstv.unimelb.edu.au/WWWDOCS/SVIMRdocs/JHPSL.html
ABRF: http://www.abrf.org
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