Bill and HP Seqer's:
Regarding your request for additional information about the Lys recovery
problem, attached are RY plots (PDF attachment) from our second HP sequencer,
that was converted to PE R1 (diluted to 3% PITC) and PE S2B late last week.
As with the first converted sequencer, a pronounced improvement in Lys
recovery was noted going from HP to PE reagents. The lot #s involved were
HP-R1: 27-mamz-m1 (different lot than 1st sequencer), HP-S2A: 26-k7yz-k9
(same lot as 1st sequencer, but more recently received), PE-R1: 9902020 and
PE-S2B: 9902040.
Since we have seen the benefit of changing from S2A to S2B on two
sequencers and since the same S2A batch was involved in each case, S2A lot #
26-k7yz-k9 seems to have a problem and other S2A batches may be similarly
effected. Please post a RY plot showing the Lys recoveries for this batch as
seen by your QA group or a more recent analysis of packaged S2A.
It is unfortunate that you were unaware of or did not receive the copy
of my original note that was blind copied to you so as to avoid undue
attention or ridicule on your part. It came to my attention over the weekend
that the idiosyncracies of some e-mail handling programs allow the delivery
of BCC'ed e-mail, but not the BCC'ed address info to the BCC receiver. To
aid you in identifying the appropriate copy of the original posting, this
note has been both CC'ed and BCC'ed to your e-mail address.
Because the apparently defective bottles of S2A were almost consumed, we
have not contacted HP for replacements, but we are hesitant to use the
remaining four unopened bottles of S2A from the same lot. Since the S2A
issue may require some time to resolve and since other S2A lots may be
problematic, we have restocked with sufficient S2B to cover our interim needs.
Please let us all know when the S2A problem has been corrected so that
we can resume our S2A purchases and pursue replacements of existing stock on
hand.
Many Thanks,
Steve
====================
Stephen Tindall, Ph.D.
Argo BioAnalytica, Inc.
====================
Subj: HP Seq: Lys Recovery Problem (PDF Figs. Attached)
Date: 4/30/99 6:52:02 PM Eastern Daylight Time
From: BILL_GETTE@HP-PaloAlto-om4.om.hp.com
Sender: abrf-request@aecom.yu.edu (Association of Biomolecular
Resource Facilities)
To: abrf@aecom.yu.edu (Recipients of ABRF List)
CC: abrf@aecom.yu.edu
Dear Steve:
I am writing this note in response to your e-mail posting on the ABRF
web from earlier in the week. There are a few issues that we can
clarify for you, but more information from you would help us a lot.
This is the first instance of the reduced Lys problem we have heard of.
Have you contacted your local HP representative to inform them of this?
If not, would you like to escalate this issue so that we can properly
identify the problem and help overcome any difficulties you have
experienced? To do this, please contact HP by calling the following
telephone number: 1-800-424-9759. Alternatively you can contact myself
here at Bioscience Products. We would like to help you resolve your
issues.
With regard to the contaminant in the S2A bottle, we are surprised by
this information. The plastic coating on the bottles has been the cause
of particulate matter in the past. However, we have undergone great
pains to improve our quality control and take great care in our bottle
cleaning and inspection process to avoid contaminated bottles being
delivered to our customers. Our inspections are done prior to labeling
the bottle. The bottle that contained visible contaminants should not
have been sent out. In this case, please dispose of the liquid contents
and send us back the empty, capped bottle that shows the contaminant,
with label, for further analysis. If you would like a replacement
bottle free of charge as per our 90-day replacement policy, please
enclose all relevant information including the date of receipt of this
particular bottle.
We are very sorry that you have been experiencing problems with your HP
Protein Sequencer consumables. In the future, if you would like our
assistance in resolving issues, please contact us. We acknowledge that
many subscribers to the ABRF web may have similar experiences as you,
and this may be very valuable information to all subscribers.
Hewlett-Packard monitors the ABRF for feedback regularly, but just in
case we do miss something, it would be better if you could contact us
directly and alert us to the problem. That way we can ensure we focus on
the problem at the earliest possible time.
Please accept our apologies for any inconveniences this has caused you.
If you have any further questions, please contact me.
William Gette,Ph.D.
Product Manager
Protein Sequencing Products
Chemical Analysis Group
Hewlett-Packard Company
Content-Type: application/pdf; name="1685-LYS.PDF"