RE: DNA SEQ- Accutrac - any experience with it?
Margaret Robertson (margaret.robertson@hci.utah.edu)
Mon, 10 May 1999 09:36:29 -0600
We have been using AccuTrac for a couple of weeks now to help track our 96
lane gels. It really helps define the lanes for manual tracking or
adjustment of automated tracking, especially when more than a few lanes drop
out. I don't see any indication that it helps with automated tracking yet.
We're going to send some data back to CBI to help them refine the marker. We
resuspend our samples in 3ul of marker dye and load 1ul. We load the marker
dye in every well of a 96 lane gel, loading odd and even lanes 3 minutes
apart. You could just use the marker in odd OR even lanes, but it works out
to be relatively inexpensive per well so we define all 96 lanes. It's worth
the cost just to be sure of lane tracking in a core laboratory where you
have the potential for multiple lane drop outs. Give it a try!
Margaret Robertson
Director, DNA Sequencing Facility
University of Utah,
4A 432A, SOM
50 North Medical Drive,
Salt Lake City, UT 84132
Tel: 581-4736
Fax: 585-2978
margaret.robertson@hci.utah.edu
http://www.hci.utah.edu/groups/sequencing