Dear ABRF:ers
I am running a 494 Procise Sequencer and I have trouble with
the PTH separation. Please have a look at the attached chromatogram.
I would bee very happy if somebody could figure out what's wrong.
I have changed all the pump seals. Solvents A , B and Premix tested.
New column and precolumn installed. The chromatograms look the same through
the whole testing procedure; a big slope in the beginning and the
hydrophobic PTHs elute too early.I am getting desperate.
Thanks in advance.
--============_-1285701221==_============
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Content-Type: text/plain; charset="us-ascii"
Christer Wernstedt
Ludwig Institute for Cancer Research
Box 595
S-75124 Uppsala
Sweden
Phone: +46-18-160424
Fax: +46-18-160420
E-mail christer.wernstedt@licr.uu.se
Home page http://www.licr.uu.se
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