Biotin will block the sequence if it is on the N-terminal of the protein,
however, if the N-terminal is not biotinylated, the protein will sequence
right through any biotin label that may be present without any problems.
You can get more information from the following :
Smith, J.S., Miller, B.T., Knock, S.L., and Kurosky, A. Biotinylated
peptides/proteins: I. Determination of stoichiometry of derivatization.
Analyt. Biochem. 197:247-253, 1991.
Smith, J.S., Miller, B.T., and Kurosky, A. Biotinylated peptides/proteins:
II. Identification of biotinylated lysyl phenylthiohydantoins. Analyt.
Biochem. 197:254-257, 1991.
Good luck,
John S. Smith
Protein Chemistry Lab
UTMB, Galveston
-----Original Message-----
From: Amanda Hall
To: Recipients of ABRF List
Sent: 5/12/99 12:43 AM
Subject: ProtSeq: biotin-labelled proteins
Hi everyone,
A customer rang me to ask if biotin labelled proteins could be
sequenced.
I have no idea and so would appreciate your input. Can it be done? Does
anything special need to be done before starting sequencing?
Thanks in advance
Amanda Hall
_______________________________________________
_______________________________________________
Amanda Hall (BSc. Hons)
Professional Officer
Newcastle Protein
University of Newcastle
ph 02 4921 7299
fax 02 4921 6903
email: mbah@mail.newcastle.edu.au
______________________________________________
______________________________________________