Re: Protseq PTH separation

Jose Cesar Rosa (jcrosa@beverly.fmrp.usp.br)
Wed, 12 May 1999 16:10:12 -0300 (EST)

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In reply to: Christer Wernstedt: "Protseq PTH separation"

Dear Christer,
Last year we had a similar separation problem, all PTH-AA eluted
off earlier. Bob Apfelzweig from ABI told me that this problem occurs
when the column was stored for more than 6 months prior to installation on
the Procise, and he sent me a installation procedure which have been
working and preventing this phenomenon called "channeling" (new channels
on the column resin bed).
Starting equilibrate de column in high organic solvent
concentration (90-95% solv B at low flow rate 20-50ul/min for several
hours), and gradually increase the flow rate to 300ul/min, after this
deacrease in 10%B stepwise from 90% untill the initial conditions. The
entire procedure could take several hours.
I hope this information could be useful
Best Regards,

Cesar

Jose Cesar Rosa, Ph.D
Centro de Quimica de Proteinas
Faculdade de Medicina Ribeirao Preto
Universidade de Sao Paulo
Av. Bandeirantes, 3900
14049-900 Ribeirao Preto, Brazil
phone: 55-16-6331884
fax: 55-16-6332119
email: jcrosa@fmrp.usp.br

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In reply to: Christer Wernstedt: "Protseq PTH separation"