Maria-
We have had excellent results sequencing GC rich template using ABI's=20
dGTP
kit. For an example, please see my web site:
www.omrf.ouhsc.edu/OMRF/Core/DNASEQ/gc_rich.html
Sorry, I don't have advice for amplification.
Sheryl Christofferson
OMRF DNA Sequencing Facility
Oklahoma City, OK
(405)271-7393
Maria Lorenzi wrote:
> Hi,
> I would like to know if there are particular procedures to follow to
> amplificate a DNA rich in GC and to get a sequence of it.
> Could you give to me some advises?
> Thank you very much
> Maria Lorenzi
>
> "Soltanto una cosa =E8 veramente importante da fare; ho cercato ma
> non sono riuscita a fartela vedere. So comunque che un giorno la=20
vedrai
> perch=E9, come il sole, =E8 per tutti"
>
> M.L.
>=20
************************************************************************
*
> Dott.ssa Maria Lorenzi
> Dottoranda in Biochimica e Biofisica
> Istituto di Biochimica
> Facolt=E0 di Medicina e Chirurgia
> Facolt=E0 di Agraria
> Universit=E0 degli Studi di Ancona
> Via Ranieri 60131 Ancona, Italia
> Tel(lab):+39 71 220 4395
> +39 71 220 4681
> +39 71 220 4677
> Fax: +39 71 280 2117
> e-mail:=20
mlorenzi@ascu.unian.it
>=20
************************************************************************
Sheryl:=20
We used the ABI's dGTP kit to sequence several DNA fragments with high=20
GC content (for example a row of 12 Cs) and we got the same result=20