Re: Mapping disulfide bridges with Edman?

(ctriley@jhmi.edu)
Thu, 13 May 1999 10:04:59 -0400

In 1984 we determined the disulfide linkage in Insecticyanin by digesting
the thoroughly denatured apo-protein with trypsin. The four cys-containing
peptides of the reduced and alkylated digestion disappeared and two new
peptides appeared. The new peptides are sequenced, each giving the dual
sequences of their component peptides.

Riley et al., 1984, _Journal of Biological Chemistry_, 259, pp 13159-13165.

Clark T. Riley
ctriley@jhmi.edu
http://riley.bs.jhmi.edu
(410) 955-7897
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From: Mark Garfield <MGARFIELD@niaid.nih.gov>
Date: Wed, 12 May 1999 14:25:41 -0400
Subject: Mapping disulfide bridges with Edman?
To: Recipients of ABRF List <abrf@aecom.yu.edu>

Is there a way, using conventional Edman sequencing, to map disulfide
bridges in a protein? Today an investigator indicated to me that there may
be.

Thank you in advance for your consideration of my question.

Mark K. Garfield
Structural Biology Section,OSD, NIAID, NIH
Twinbrook II, room 225
12441 Parklawn Drive
Rockville, MD 20852
301-496-3786
email: MG102K@NIH.GOV

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