Re: GC content of DNA.

HOSSEINI@gmm.gen.emory.edu
Fri, 14 May 1999 9:46:49 EST5EDT

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Folks: I am sorry for incomplete posting. I think our email system was not functioning properly.
So I will try again:

Sheryl:

We used the ABI's dGTP kit to sequence several DNA fragments with high
GC content (for example a row of 12 Cs) and we got the same results as the conventional BigDye
kit.
I tried several samples and didn't see any difference. I saw your data and they were great.
Is there any particular way that this kit should be handled in order to read through GC content?

Thanks

Seyed Hosseini

Seyed H. Hosseini, Ph.D.

DNA sequencing lab
1462 Clifton Rd. Rm#416
Center for Molecular Medicine
Emory University
Atlanta, GA 30322
Tel # 404-727-1353
Fax # 404-727-8367
hosseini@gmm.gen.emory.edu

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Maybe in reply to: Maria Lorenzi: "GC content of DNA."