Paul Morrison
Molecular Biology Core Facilities
Dana-Farber Cancer Institute
Jimmy Fund Two
44 Binney Street
Boston, MA 02115
paul_morrison@dfci.harvard.edu
http://mbcf.dfci.harvard.edu
617-632-3082
fax 632-4814
Leviten, Dina wrote:
>Lisa,
>
>We just got our 3700 installed last week and here's a breif synopsis of how
>things are going. Bottom line, machine is great--software needs help.
>
>The install went well and the instrument works great. For long reads, it
>takes 4.5 hrs (of that 2.5hrs are collection of data). Using the machine is
>easy and is sure beats pouring and loading gels. The software right now is
>another story! It is pretty intuitive, but in these early stages, there are
>tools that need to be added to make our lives easier. Most of the big
>problems we have should be easily fixed with some software changes. The
>biggest problems are sample sheet preparation and printing. At this point
>it is tedious, but we have come up with a couple work-arounds that have cut
>down on the time. We are getting a database system that should address most
>of the sample sheet and printing problems.
>
>Data looks good. In the run time we get about 700 bases. Clean DNA is key.
>
>Data files are in "dos" but can be converted to "abi1" with a program called
>"SetItsType"ver1.3 (found free on the internet). This enables programs like
>Sequencher to see the chromatograms.
>
>Overall, we are really happy with our purchase and everyone loves NO
>BACKLOG!!! We are exclusively using the 3700, where we were using 2 stretch
>373's. The 3700 can run 5 96-well plates in a 24hr period, where you run
>one during the day and can set up the other 4 for an overnight run.
>
>If you want the nitty-gritty details, I can supply them, but by the time you
>would get an instrument, these problems will probably be gone (I'm
>optimistic!)
>
>Hope this helps!
>Sincerely,
>Dina Leviten
>ICOS, Corp.
>Bothell, WA
>
>
>> ----------
>> From: lisa bibbs
>> Sent: Thursday, May 13, 1999 5:03 PM
>> To: Recipients of ABRF List
>> Subject: DNASeq: New 3700?
>> >> Does anyone have one and would like to comment? We have reached are
>> capacity and are trying to decide about next years budget.
>> >> Thanks in advance
>> Lisa
>> >> >
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