RE: MALDI matrices

Amina S. Woods (amina@welch.jhu.edu)
Mon, 17 May 1999 09:57:23 -0400

I have no experience with heparin. However we've had good results for
oligosaccharides with gentisic acid and ferrulic acid depending on the
compound (saturated solutions in 50 % ethanol). 6-Aza-2-thiothymine (make
a saturated solution in 50% acetonitrile, then take the supernatant and
add 5 % ammonium citrate). This last one works well with any negtively
charged compound.

Amina

Amina S. Woods, Ph.D.
Johns Hopkins School of Medicine
725 N Wolfe street, Baltimore MD 21205
Tel: (410)614-4981, Fax: (410)955-4320

-----Original Message-----
From: Emily Hargreaves [SMTP:ejh36@mole.bio.cam.ac.uk]
Sent: Monday, May 17, 1999 3:52 AM
To: Recipients of ABRF List
Subject: MALDI matrices

I have some samples of heparin to analyse by MALDI-TOF and would be
grateful
if anyone could suggest a suitable matrix (matrices) for this analysis.

Many Thanks

Emily Hargreaves
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Emily J. Hargreaves
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