You might want to check out the following journal article:
"Utility of non-covalent complexes in the matrix-assisted laser desorption
ionization mass spectometry of heparin-derived oligosaccharides"
Juhasz and Biemann, Carbohydrate Research 270 (1995) 131-147.
This article describes the use of synthetic basic peptides for complexing to
heparin oligosaccharides to facilitate more efficient ionization of the
oliogosaccharides. They also describe the use of angiogenin, a heparin binding
protein, for the same purpose.
Good luck!
Steven Hall
Applications Specialist
PE Biosystems
850 Lincoln Centre Drive
Foster City, CA 94404
phone: (650) 554-2369
FAX: (650) 554-2345
email: hallsc@pebio.com
Emily Hargreaves <ejh36@mole.bio.cam.ac.uk> on 05/17/99 12:51:35 AM
To: Recipients of ABRF List <abrf@aecom.yu.edu>
cc:
Subject: MALDI matrices
I have some samples of heparin to analyse by MALDI-TOF and would be grateful
if anyone could suggest a suitable matrix (matrices) for this analysis.
Many Thanks
Emily Hargreaves
--------------------------------------------------------------------------
Emily J. Hargreaves
Department of Biochemistry
University of Cambridge
Tennis Court Road
Old Addenbrookes Site
Cambridge CB2 1GA UK
Tel: 01223-333638/766039
Fax: 01223-766002
International: +44-1223-333638/766039 (fax:-766002)
http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html
http://www.bio.cam.ac.uk/dept/biochem