Re: IPGphor users

Katheryn Resing (Katheryn.Resing@Colorado.EDU)
Mon, 17 May 1999 09:08:59 +0000

We couldn't get good results with a constant current focusing. We use the
programable power supply from pharmacia, at constant voltage, but varying
during the focusing in steps. I have included below the program we use,
with the instrument settings (amps and watts are limits, not constant
settings).
As long as this is up for discussion, I have a query for the other
users. We have had problems getting reproducible focusing. Strips treated
identically and focused at the same time will show nice, tight spots on
most of the strips, but about 1 out of 4 will have streaking on the higher
mass spots that looks like they haven't focused well.
Any one found a solution to that one, yet?

Weather inconsequential: afternoon thunderstorms starting, but beautiful
when its sunny, still getting occasional frost in AM and snow in foothills.
Lilacs are starting to bloom,

Katheryn Resing

I-D Focusing Parameters for four strips:
Program for Immobiline DryStrip pH 3-10L, 110mm
Phase Voltage mA W Time (hr) Vh
1 300 1 5 0.01 1
2 300 1 5 4.5 1350
3 2000 1 5 5 5750
4 2000 1 5 6.5 13000
Total: 16 20100

Program for Immobiline DryStrip pH 3-10L, 180mm
Phase Voltage mA W Time (hr) Vh
1 500 1 5 0.01 1
2 500 1 5 3 1500
3 3500 1 5 5 10000
4 3500 1 5 12.5 43750
Total: 20.5 55250