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</smaller></fontfamily><fontfamily><param>New_York</param><bigger>Dear
ABRF Colleagues,
I have a large substrate digested by an enzyme with unknown specificity
and have found several MWs of the peptides generated. I need to know
where the cleavages occurred.
1 Does anyone know if there is a program that will allow me to enter
the sequence of a substrate and the MW of the peptide(s) generated and
then show the possible cleavage points?
2 Does anybody have any ideas on what a +136 series could be? A
fraction that was supposed to contain a fluorescent dye labeled peptide
from a reverse phase HPLC run gave this +136 series envelope. I believe
the mobile phase was TFA/acetonitrile/water.
Thanks in advance.
Dick
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