Re: MS - 2?s: program to determine cuts? / 136 series?

John Cottrell (jcottrell@dial.pipex.com)
Tue, 18 May 1999 08:45:41 +0100

GPMAW should do the job. You can enter a protein sequence then search for the peptides which match a given mass within a specified a mass tolerance. If it's a large protein, you'll find a lot of matches for each MW unless you have very accurate mass data.
URL is http://welcome.to/gpmaw

Richard F. Cook wrote:

> Dear ABRF Colleagues,
>
> I have a large substrate digested by an enzyme with unknown specificity and have found several MWs of the peptides generated. I need to know where the cleavages occurred.
>
> 1 Does anyone know if there is a program that will allow me to enter the sequence of a substrate and the MW of the peptide(s) generated and then show the possible cleavage points?
>
> 2 Does anybody have any ideas on what a +136 series could be? A fraction that was supposed to contain a fluorescent dye labeled peptide from a reverse phase HPLC run gave this +136 series envelope. I believe the mobile phase was TFA/acetonitrile/water.
>
> Thanks in advance.
>
> Dick

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John Cottrell
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