> I've just started making my own IPG strips to solve the problem of
> inconsistent strips and focusing. I have run about a dozen 2D gels so far
> and all gels show consistent spot quality and pattern. The spot quality
and
> pattern of gels using labmade strips are the same (maybe even a little
better)
> as the commercial strips. It only takes about 2 hours to set up and pour
> the gel for IPG strips. One IPG slab can make about 30 strips (at about
50
> cents per strip). Cutting the strips precisely is still a challenge.
>
I remember Novex had a great slicer for making uniform nylon membrane strips
from a larger blot. You might check with them to see if their system can cut
a support and gel like yours.
Walt Schick
BioSepCo