We generally perform HPLC and analysis by mass spectrometry wirth very
complex mixtures of peptides from human body fluids. Most of our experien=
ce
is based on reversed phase and cation exchange chromatography. Experience
with phoshopeptides is not that high as they do not occur too often in
extracellular fluids.
You might look at
M. J=FCrgens, M. Schrader, M. Raida, W.-G. Forssmann and P. Schulz-Knappe
=93Multi-dimensional mapping of human blood peptides by mass spectrometry=
.=94 J.
Biol. Techniques 9 (1998) 24-30 (www.abrf.org/JBT/ 0006).
Schulz-Knappe, P., M. Schrader, L. St=E4ndker, R. Richter, R. He=DF, M. J=
=FCrgens
and W.-G. Forssmann (1997). =93Peptide bank generated by large-scale
preparation of circulating human peptides.=94 J. Chromatogr. A 776: 125-1=
32.
Most of the members of our team derive from the Lower Saxony Institute fo=
r
Peptide Research, therefore we have years of experience in that field. We
perform R&D to find new disease markers and lead compounds, but also prov=
ide
contract analysis and consulting in the field of peptide proteomics. For
more information, visit our homepage at www.biovision.de.
Dr. Michael Schrader (CEO)
----------------------------------------
BioVisioN GmbH & Co. KG
Feodor-Lynen-Str. 5
D-30625 Hannover
GERMANY
Phone +49-511-53 88 96-12
Fax +49-511-53 88 96-66
Internet www.biovision.de
E-mail m.schrader@biovision.de
-----Urspr=FCngliche Nachricht-----
Von: Ulrich Schneider <schneideru@ubaclu.unibas.ch>
An: Recipients of ABRF List <abrf@aecom.yu.edu>
Datum: Dienstag, 18. Mai 1999 13:48
Betreff: HPLC
>Dear colleagues,
>does anyone have some experience with (an)ion-exchange HPLC of peptides
>coming from crude digests? I'am especially interested in separating
>phosphopeptides (i.e. acidic ones), which were originally derived from V=
ERY
>complex mixtures.
>Any suggestions/references welcome!
>
>Regards
>Dr. Ulrich Schneider
>
>---------------------------------------
>Ulrich Schneider, Ph.D.
>Biocenter of the University of Basel
>Dept. for Biochemistry, R578
>Klingelbergstr. 70
>CH-4056 Basel / Switzerland
>Phone: +41 (61) 267 2169/68
>Fax: +41 (61) 267 2148
>eMail office: schneideru@ubaclu.unibas.ch
>---------------------------------------
>
>
>