Re: MS - 2?s: program to determine cuts? / 136 series?

Luisa Rusconi (Luisa.Rusconi@eu.pnu.com)
Wed, 19 May 1999 09:48:35 +0200

Dick

there was an exhaustive answer for your second question, sent by
Beverly DaGue to another ABRFer on July 7, 1998. Retrieving it in ABRF
archive should be possible (unfortunately I have only archived it as
the hardcopy), however, these +136 series of peaks appeared to be
sodium trifluoroacetate clusters (with the first ion of the series at
159 amu). This may generally be due from poor quality water, or
contaminated glassware, or alkaline reagents stored in glass bottles.
Bye!

Luisa
---------------------------

Luisa Rusconi
Pharmacia & Upjohn
Dept. of Biology
I-20014 Nerviano (MI)
+39-02-48383801
+39-02-48383750 fax
luisa.rusconi@eu.pnu.com

______________________________ Reply Separator _________________________________
Subject: MS - 2?s: program to determine cuts? / 136 series?
Author: "Richard F. Cook" <cook@MIT.EDU> at Internet-europe
Date: 5/17/99 8:51 PM

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</smaller></fontfamily><fontfamily><param>New_York</param><bigger>Dear
ABRF Colleagues,



I have a large substrate digested by an enzyme with unknown specificity
and have found several MWs of the peptides generated. I need to know
where the cleavages occurred.


1 Does anyone know if there is a program that will allow me to enter
the sequence of a substrate and the MW of the peptide(s) generated and
then show the possible cleavage points?


2 Does anybody have any ideas on what a +136 series could be? A
fraction that was supposed to contain a fluorescent dye labeled peptide
from a reverse phase HPLC run gave this +136 series envelope. I believe
the mobile phase was TFA/acetonitrile/water.


Thanks in advance.



Dick

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