Re: DNASeq: T signal strength problem
Robert Lyons (boblyons@umich.edu)
Wed, 19 May 1999 08:19:37 -0400
Kevin Hayes wrote:
>
> In our high-throughput sequencing, we have encountered a problem
> pertaining to our T signal strength. The peak height of the Ts is
> roughly 1/10th of the other bases. The base-calling software has
> problems calling any Ts. This is a lab-specific problem and has occurred
> using both bigdye and ET chemistries and with non-highthroughput
> sequencing. Has anyone heard of this and if so, where did our Ts go?
>
> Kevin Hayes
> krhayes@students.wisc.edu
> University of Wisconsin
Just for kicks, ask the lab whether they use some procedure that
leaves excess residual dTTP in the DNA prep. This would produce
exactly the syumptoms you describe. Problem is, I can't think of
any reason they might have had only an excess of T.
Bob Lyons
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Robert Lyons, Ph.D.
Director, DNA Sequencing Core
University of Michigan
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