http://www.abrf.org/archives/hmail/9824/0030.html
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Dirk......
'Electronic Discussion Group Archives'
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At 7:48 AM -0000 5/19/99, Luisa Rusconi wrote:
> Dick
>
> there was an exhaustive answer for your second question, sent by
> Beverly DaGue to another ABRFer on July 7, 1998. Retrieving it in ABRF
> archive should be possible (unfortunately I have only archived it as
> the hardcopy), however, these +136 series of peaks appeared to be
> sodium trifluoroacetate clusters (with the first ion of the series at
> 159 amu). This may generally be due from poor quality water, or
> contaminated glassware, or alkaline reagents stored in glass bottles.
> Bye!
>
> Luisa
> ---------------------------
>
> Luisa Rusconi
> Pharmacia & Upjohn
> Dept. of Biology
> I-20014 Nerviano (MI)
> +39-02-48383801
> +39-02-48383750 fax
> luisa.rusconi@eu.pnu.com
>
>
>______________________________ Reply Separator
>_________________________________
>Subject: MS - 2?s: program to determine cuts? / 136 series?
>Author: "Richard F. Cook" <cook@MIT.EDU> at Internet-europe
>Date: 5/17/99 8:51 PM
>
>
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></smaller></fontfamily><fontfamily><param>New_York</param><bigger>Dear
>ABRF Colleagues,
>
>
>
>I have a large substrate digested by an enzyme with unknown specificity
>and have found several MWs of the peptides generated. I need to know
>where the cleavages occurred.
>
>
>1 Does anyone know if there is a program that will allow me to enter
>the sequence of a substrate and the MW of the peptide(s) generated and
>then show the possible cleavage points?
>
>
>2 Does anybody have any ideas on what a +136 series could be? A
>fraction that was supposed to contain a fluorescent dye labeled peptide
>from a reverse phase HPLC run gave this +136 series envelope. I believe
>the mobile phase was TFA/acetonitrile/water.
>
>
>Thanks in advance.
>
>
>
>Dick
>
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>
>
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