From: Jeff Livingstone@WATTS WATERS SALES on 05/19/99 05:03 PM
To: abrf@aecom.yu.edu
cc:
Subject: SDS in 2D-PAGE gels for MS
I am currently evaluating the market interest of a new compound we have
recently created and patented, an acid-labile surfactant (ALS) analogue to
SDS. This ALS material is primarily for use in the 2D-PAGE gel separation
and subsequent ESI-MS detection of proteins and peptides. From our
studies, it appears we can achieve a minimum of a 10-fold enhancement of
signal sensitivity when using the ALS, as opposed to SDS, in our gels and
buffers.
I am eager to get the ABRF community's perspective on the potential of this
new product, and to locate people who might be willing to beta-test this
product for us.
Some research has provided the following general points:
1) About one-half of the people I have spoken to said that SDS was a big
problem in their use of MS for protein and proteomics work. The other half
said by the time they get to the MS stage, the SDS is so dilute from
unrelated sample preparation and handling, that it poses little problem for
MS resolution.
2) Most MALDI users use Millipore ZipTips, or home-made reversed-phase
micro columns (with ODS-AQ or POROS R1, for example) for removing salts
and SDS from their samples prior to MS. The majority of the people who use
ZipTips do not like them, for various reasons (e.g. large volumes, excess
channeling, slow equilibrium leading to multiple pipetting steps, low
sample recovery).
3) Most ESI users simply create an in-line RP column to desalt and de-SDS
prior to ESI.
4) The Proteomics community would love to be rid of Lammeni gels,
completely.
Feedback, folks?
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