Hi Jeff
Hi ABRFers
Besides using reverse phase sorbents for the retention of SDS which is
not too successful, several vendors including us offer small pre-columns
for capillary or micro LC packed with a special sorbent for the removal
of detergents like SDS.
A paper pertaining to the issues of ionic detergent removal:
J.P.C. Vissers, W.P. Hulst, J.P. Chervet, H.M.J. Snijders, and C.A.
Kramers
Automated On-line Ionic Detergent Removal from Minute Protein/Peptide
Samples Prior to Liquid Chromatography-Electrospray Mass Spectrometry
Journal of Chromatography B, 686 (1996) 119-128
If you have no access to this paper, you can e-mail me directly for a
reprint.
Best regards:
Jean-Pierre
******************************************************
Jean-Pierre Salzmann
Project Manager
LC Packings (USA) Inc.
80 Carolina Street
San Francisco, CA 94103
Phone (415) 552-1855
Fax (415) 552-1859
E-mail: jpsalzmann@earthlink.net
www.lcpackings.com
*******************************************************