Removal of non-ionic detergent (fwd)

Dirk Krapf (krapf@salt2.med.harvard.edu)
Fri, 21 May 1999 12:25:32 -0400 (EDT)

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---------- Forwarded message ----------
Date: Wed, 19 May 1999 23:38:12 +0100
From: Dougie Lamont <dougie01@globalnet.co.uk>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Subject: Removal of non-ionic detergent

Hi all,

Does anybody have any experience with non-ionic detergent removal. I use it in a method to de-glycosylate my protein but need to get rid of it prior to ESI-MS. I've tried RPC C-18 but the non-ionic detergent elutes all over the gradient. I've also tried the NID traps from Michrom. They appeared to work for apomyoglobin but did not work for my protein (previously reduced and alkylated).I'm about to try the HILIC spin columns from AmiKa. Has anybody tried these ?. Any information, experiences would be appreciated.

regards

Dougie Lamont

PPL Therapeutics

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Hi all,
 
Does anybody have any experience = with non-ionic=20 detergent removal. I use it in a method to de-glycosylate my protein but = need to=20 get rid of it prior to ESI-MS. I've tried RPC C-18 but the non-ionic = detergent=20 elutes all over the gradient. I've also tried the NID traps from = Michrom. They=20 appeared to work for apomyoglobin but did not work for my protein = (previously=20 reduced and alkylated).I'm about to try the HILIC spin columns from = AmiKa. Has=20 anybody tried these ?. Any information, experiences would be=20 appreciated.
 
regards
 
Dougie Lamont
 
PPL = Therapeutics
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