Karla
If your hemoglobin is being reduced in the sample well but becoming
reoxidised in the stacker gel, you might prevent this by adding 2mM
mercaptoacetic acid to yout upper well buffer. This will track ahead of
your protein and maintain a reducing environment. Worth a try.
Len
*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html