Juan,
Another method that is even faster than tea bags is to use
polypropylene syringes anywhere from 5 to 20+ mL size (like they
use in hospitals) with a poly prop. frit in the bottom. That is,
if you still want to do hand style synthesis.
You simply pull the reaction solutions (made up in a 50 mL conical)
and rinses up with the plunger, add a small air slug to give mixing
space, run your hold times (on an orbital shaker I might add...),
and then push out the solution repeating as necessary until the
sequence is made.
I have made up to 14 completely different sequences at once from 5
to 15+ residues anywhere from 1 to 3 days, depending on the
coupling efficiency, by FMOC with HOBT/TBTU/DIEA using this
method. You can also pull small samples easily for Kaiser testing
by removing the plunger and getting a few beads of resin for a
simple 'go/no go' result on the coupling. When done, you can dry
the resin in the syringe and even do the cleavage in there. No
muss, no fuss. Just make sure you pick a syringe size that'll hold
all the resin and reaction medium.
As far as the frits, you'll need cork borers to cut them to
size...they should be tight in the bottom of the syringe and not
rise when you pull up your soln's...as to a source of the material,
you'll probably need to look locally for it. We used to get the
stuff from a local supplier here in the N.E. USA in sheets (2-3mm
thick) that could easily be punched to size.
Hope this helps,
Steven Johnson
Steven.Johnson@Biomeasure.com
______________________________ Reply Separator _________________________________
Subject: Peptide synthesisers
Author: Juan Cooper <juanC@qimr.edu.au> at Internet
Date: 5/20/99 5:29 PM
Our group presently synthesises peptides by the Tea bag method which is
very cost effective but time between batches is an issue. If an urgent
one-off peptide is requested time is a problem.
Can anyone recommend an automated peptide synthesiser that satisfies the
following requirements:
Tboc or fmoc chemistry
single column (cheaper than multiple column)
100 mg resin scale
User supplied (prepared) reagents such as amino acids, activators etc
Flexibility to use coupling chemistries like in situ activation HBTU/DIEA
Any comments will be greatly received.
Thanks
Juan
************************************************************************
****************
Dr. Juan A. Cooper Laboratory Manager - Scientific Support
Services
The Queensland Institute of Medical Research Phone : 61 7 3362 0210
The Bancroft Centre, P.O. Royal Brisbane Hospital Fax : 61 7 3362 0111
Brisbane, QLD 4029 AUSTRALIA E-mail: juanC@qimr.edu.au