> hi all,
>=20
> thanks for the replies to my question of how to get rid of non-ionic
> detergents. I've found in the past that one round of chloroform/methano=
l
> precipitation (wessel and fl=B3gge) was enough to get rid of the SDS bu=
t not
> the non-ionic detergent. Maybe its the way I do it !!. The way I solved=
it
> was to run the material on SDS-PAGE gel first, then do negative stainin=
g,
> excise the band and elute (castellanos-serra et al) prior to chloroform=
:
> methanol. It worked !! .
>=20
> Does chloroform :methanol precipitation normally get rid of non-ionic
> detergents along with SDS?
>=20
Would you mind summarising the replies? If they went through the=20
listserver I missed them somehow
Patrick