dnasyn: wasting amidite/expedite

Michael A Imbalzano (imbalzan@SAS.UPENN.edu)
Mon, 24 May 1999 08:53:19 -0400 (EDT)

Hi,

It seems that every time I setup an Expedite/Moss to run overnight
I often find there is an amidite bottle that is 1-2ml shy of the
required amount needed to complete the run. Sometimes I "pour in" another full
bottle, other times I just dump the old amidite into the waste container.

As summer approaches an the humidity rises here in Philadelphia I am more
hesitant to combine bottles. At the same time it seems like a huge waste to
keep pouring good chemicals down the drain.

I usually run through a 2 gram (40mls of amidite) bottle in about a week.
If we were using a larger bottle I wouldn't mind dumping 5-10mls at the end.
I know that PerSeptive experimented with 5 gram bottles but the "bottle upgrade"
never got off the ground.

Does anyone else have this problem?
Has anyone modified their instrument to use larger bottles?

Thanks,

Mike Imbalzano
imbalzan@sas.upenn.edu