Re: dnasyn: wasting amidite/expedite

Linda K. Beggerly (lkb7h@unix.mail.virginia.edu)
Wed, 26 May 1999 08:54:55 -0400 (EDT)

Michael,
You did not specify which expidite you have, 8905 or 8909. If you
have an 8909, consider using one of the extra amidite vessels for a second
bottle. When the normal position is empty you can transfer remaining
amidite to the correct position. Of course you must designate bottle 5
for a specified number of positions. Not an ideal solution, but it may
help.

On Mon, 24 May 1999, Michael A Imbalzano wrote:

> Hi,
>
> It seems that every time I setup an Expedite/Moss to run overnight
> I often find there is an amidite bottle that is 1-2ml shy of the
> required amount needed to complete the run. Sometimes I "pour in" another full
> bottle, other times I just dump the old amidite into the waste container.
>
> As summer approaches an the humidity rises here in Philadelphia I am more
> hesitant to combine bottles. At the same time it seems like a huge waste to
> keep pouring good chemicals down the drain.
>
> I usually run through a 2 gram (40mls of amidite) bottle in about a week.
> If we were using a larger bottle I wouldn't mind dumping 5-10mls at the end.
> I know that PerSeptive experimented with 5 gram bottles but the "bottle upgrade"
> never got off the ground.
>
> Does anyone else have this problem?
> Has anyone modified their instrument to use larger bottles?
>
> Thanks,
>
> Mike Imbalzano
> imbalzan@sas.upenn.edu
>
>