Michael has the 8909, because the 8905 is not upgradable with MOSS.
Transferring to the second row of bottles in the middle of a MOSS run will
require calculation and manual replacement of one (or more) characters with
numbers within a good number of sequences, therefore is not very convenient.
I would suggest a different approach:
Bear in mind that the protocols for ABI synthesizers give you the choice of
diluting your amidites in two different amounts of ACN, and the difference
is 2-FOLD (!). Extrapolating from this, you should be able to dilute 1 g
amidite in 25 ml ACN (instead of 20) and 2 g in 50 ml (instead of 40). The
quality does not suffer (judging from the trityls and the OD260). That is,
if you are operating at higher capacity, have a fast turnaround of the
amidites, and if your source of amidites is reliable. At that point the 10
ml that you pour down the drain from the 50 ml bottle are the 10 ml extra
that you add at the beginning.
Well, human nature being what it is, it still hurts to see the 10 ml going
down the drain, even if your ancestors are not from Aberdeen...
wagner
----- Original Message -----
From: Linda K. Beggerly <lkb7h@unix.mail.virginia.edu>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Cc: Recipients of ABRF List <abrf@aecom.yu.edu>
Sent: 26 mai, 1999 08:54
Subject: Re: dnasyn: wasting amidite/expedite
> Michael,
> You did not specify which expidite you have, 8905 or 8909. If you
> have an 8909, consider using one of the extra amidite vessels for a second
> bottle. When the normal position is empty you can transfer remaining
> amidite to the correct position. Of course you must designate bottle 5
> for a specified number of positions. Not an ideal solution, but it may
> help.
>
>
> On Mon, 24 May 1999, Michael A Imbalzano wrote:
>
> > Hi,
> >
> > It seems that every time I setup an Expedite/Moss to run overnight
> > I often find there is an amidite bottle that is 1-2ml shy of the
> > required amount needed to complete the run. Sometimes I "pour in"
another full
> > bottle, other times I just dump the old amidite into the waste
container.
> >
> > As summer approaches an the humidity rises here in Philadelphia I am
more
> > hesitant to combine bottles. At the same time it seems like a huge waste
to
> > keep pouring good chemicals down the drain.
> >
> > I usually run through a 2 gram (40mls of amidite) bottle in about a
week.
> > If we were using a larger bottle I wouldn't mind dumping 5-10mls at the
end.
> > I know that PerSeptive experimented with 5 gram bottles but the "bottle
upgrade"
> > never got off the ground.
> >
> > Does anyone else have this problem?
> > Has anyone modified their instrument to use larger bottles?
> >
> > Thanks,
> >
> > Mike Imbalzano
> > imbalzan@sas.upenn.edu
> >
> >