I always explained this to myself as a diffusion effect. The developer in
the outer parts of the gel is in better exchange with the developer in the
surrounding liquid. Thereby the silver is faster reduced in these areas
(both sides of the gel) then in the center of the gel.
Regards,
Juri
>Hi, all,
>I have a researcher here who has silver stained a gel (using the Mattias
>Mann 1996 protocol)--his band looks a bit odd (this gel is 1.5 mm thick)
>in that the staining looks like it is on the surface of both sides of the
>band but not through the thickness of the gel--has anyone seen this
>before?
>
>Thanks,
>Deb McMillen
>Institute of Molecular Biology
>University of Oregon
>EUgene OR 97403
>
>
>
Juri Rappsilber
Protein Interaction Laboratory
University of Southern Denmark