Cesar
On Mon, 7 Jun 1999, Sandy Kielland wrote:
> Hello all;
> A customer recently submitted a small peptide (MW 884) for sequence and
> amino acid analysis. I have been unable to get sequence, but the PTC
> profile shows a non-standard amino acid eluting just before arginine (ABI
> 420). The mass works out correctly if I use arginine's mass and add an
> acetyl group. The only modified amino acids I know of that elute in this
> region are met-sulfoxide, dehydroalanine and homoserine but these are
> unlikely candidates. The peptide is probably cyclic and may contain
> ornithine, beta diaminopropionic acid, isooctanoic acid or diaminobutyric
> acid. Does anyone familiar with amino acid analysis know where these
> compounds elute or is willing to take an educated guess?
> Thanks for any input,
> Sandy Kielland,
> Microsequence and peptide synthesis facility
> University of Victoria
> Victoria,B.C.
> Canada
>
>
Jose Cesar Rosa, Ph.D
Centro de Quimica de Proteinas
Faculdade de Medicina Ribeirao Preto
Universidade de Sao Paulo
Av. Bandeirantes, 3900
14049-900 Ribeirao Preto, Brazil
phone: 55-16-6331884
fax: 55-16-6332119
email: jcrosa@fmrp.usp.br