Re: PTH-Cys-CAM

John Hempel (hempel@psc.edu)
Wed, 09 Jun 1999 13:12:18 -0400

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LiFeng-
In addition to Rod Levine's comments, alpha-lactalbumin (Mr ~13kDa) offers
a quick way to determine where the peak elutes in your system The
material is cheap from Sigma (catalog L-6385) and has Cys at the 6th
position (EQLTKCEVFREL....). Just alkylate some by whatever protocol and
run it.

Regards, JH

At 02:57 PM 6/9/99 +0800, leefeng wrote:
>I have modified some of cysteine residues of a polypeptide using IAA.
>I want to know the position of labled cysteine residues ...............

>li,feng
>Biology Department
>Hunan Normal University
>changsha, hunan, China.

John Hempel, PhD Ph (412) 624 0161
University of Pittsburgh FAX (412) 624 4759
Department of Biological Sciences
Clapp Hall 301
Pittsburgh PA 15260 USA

email: hempel@psc.edu
http://www.pitt.edu/~biology/faculty/hempel.html

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