Re: ProtSeq
Gregory Grant (ggrant@pcg.wustl.edu)
Wed, 9 Jun 1999 16:39:18 -0500
>Question for ARBF'ers:
>
>I am trying to quickly remember what happens to side chain amines (such as
>beta of Dpr or epsilon of Lys) during edman degradation, for the sake of
>determining what to expect on edman products by some MS experiments we are
>going to perform.
>
>Don't they also react with the PITC to form phenylthiocarbamyl derivatives
>that don't cleave with TFA? Need your help.
>
>Thanks,
>
>Randy Wilhelm
>Mallinckrodt, Inc
>rrwilhe@mkg.com
>(314)654-3965
Yes they do. A primary amine is a primary amine. They don't cleave because
there is nothing to cyclize to or with usually.
Gregory A. Grant
ggrant@pharmdec.wustl.edu