Re: PTH-Cys-CAM

Michael Schrader (m.schrader@biovision.de)
Thu, 10 Jun 1999 17:39:36 +0200

As sequencing needs time and is quite expensive I would suggest to use a
peptide starting with Cys better than lactalbumin. There is even a CAM
peptide available at Sigma (E 9384, an EGF fragment). On the other hand all
the endothelins start with Cys.

>John Hempel <hempel@psc.edu> wrote
>LiFeng-
> In addition to Rod Levine's comments, alpha-lactalbumin (Mr ~13kDa) offers
>a quick way to determine where the peak elutes in your system The
>material is cheap from Sigma (catalog L-6385) and has Cys at the 6th
>position (EQLTKCEVFREL....). Just alkylate some by whatever protocol and
>run it.

Michael
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