Re: Myristyl group
Steven.Johnson@biomeasure.com
Mon, 14 Jun 1999 08:08:12 +0100
Terry,
It's quite simple actually...
You can add it as myristic acid and use normal coupling protocols
on the free amine of the N-terminus.
If you want it on an internal lysine, make your peptide, use
Fmoc-Lys(Dde)-OH (available from Novabiochem), and then use a
BOC-AA-OH for the last amino acid in your sequence. When finished
your assembly on the resin, treat the peptide/resin with a 2%
hydrazine/DMF solution 2 x 2 min. to remove the Dde group, wash and
then add your myristic acid with the appropriate activators. The
N-Terminal Boc group will fall off upon cleavage with TFA cocktails
so it's not a concern.
The other way is to use Myristic anhydride which only needs an
equivalent of DIEA to help along the addition, but that is more
expensive as you are throwing away half the molecule.
Regards,
Steven Johnson
Steven.Johnson@Biomeasure.com
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Subject: Myristyl group
Author: "Terry Stoming" <TSTOMING@MAIL.MCG.EDU> at Internet
Date: 6/11/99 10:27 AM
Several people have approached me concerning the addition of a myristyl group to
peptides. Does anyone have any info on how to do it.