Re: ProtSeq-ABI477 & Porton

John Hempel (hempel@psc.edu)
Wed, 16 Jun 1999 19:03:47 -0400

Hi Sandie,
At 02:56 PM 6/10/99 -0400, you wrote:
>.......on our Porton LF3000: Tyr is giving two peaks, one normal
>in position and another eluting shortly after Ala. They are about equal in
>height. Also Lys is giving two peaks ,normal (very small) and one before
>Pro. I suspect these might be PTC's? What is the most likely fix
>possibility here? Any and all help will be greatly appreciated!

*If the problem on your Porton is still unresolved, how are your glycines?
In my experience, both a LONG time ago and also last night on my Porton,
Gly is the most sensitive to non-cyclization. What I assume to be PTC Gly
runs before PTH Asp. In last night's run, it was non-delivery of the
conversion acid. I was amazed to see that all other (of about 11
different) residues except perhaps Pro were totally normal. But, there was
no Tyr in that sequence, and all my Lys were modified so I can't compare
with your results on that. BTW, the R4 pressurize valve is now fixed
(30min - can that be done on an ABI?).

Regards,
John
John Hempel, PhD Ph (412) 624 0161
University of Pittsburgh FAX (412) 624 4759
Department of Biological Sciences
Clapp Hall 301
Pittsburgh PA 15260 USA

email: hempel@psc.edu
http://www.pitt.edu/~biology/faculty/hempel.html