We QC all our oligos <35 bases by maldi.
See Ball, R.W. and Packman, L.C. (1997) MALDITOF mass spectrometry as a
rapid quality control method in oligonucleotide synthesis. Anal. Biochem.,
246, 185-194 for our methodology.
This paper was written based on a machine without delayed extraction. To
update it, on a delayed extraction MALDI 4 (Kratos) we now see resolutions
of up to 1000 at 10kDa in linear and sensitivity down to 1pmol, and masses
accurate to <0.04% with external calibration (0.01% internal). The
methodology remains the same, based on HPA and cation exchange beads. It is
very robust.
Len
At 12:14 pm +0300 16/6/99, Ulf Hellman wrote:
>Dear MALDI users!
>Can anyone please suggest a robust procedure for MALDI analysis of
>oligonucleotides and similar components. Type of matrix, type of modes
>(lin/refl, pos/neg, etc) in the instrument, sample pretreatment, solvents
>and whatever might be relevant? Realistic concentration of analyte?
>Thanks in advance. /Ulf
*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html