> Molecular Biology Core Facilities 7:24 AM
6/10/99
> Dana 830 SDS Conc. for MALDI
> 632-3082
>
>Hi All,
> Sometime in the not so distant past I remember reading an article or
someone
>mentioning something about the critical % of SDS that can be used
when
>analyzing samples by MALDI. I seem to remember what was said was that
low
>levels(<<0.01%) of SDS were fine and surprisingly levels above 1% or
2% were
>fine. Does anyone recall reading about this or am I just having some
wishful
>thinking. Thanks.
>
>Jim,
this may be what you're looking for:
"Analysis of Peptide and Protein Samples Containing Surfactants by
MALDI-MS". Amado, F.M.L., Graca, Santana-Marques, M., Ferrer-Correia,
A.J., and Tomer, K.B. (1997) Anal. Chem. <bold>69</bold>, 1102-1106.
regards,
Gary Hathaway
Gary M. Hathaway
Director, Protein Analytical Lab
Caltech, 139-74
400 S. Wilson Ave.
Pasadena, CA 91125
hathaway@cco.caltech.edu